Journal
PHYTOMEDICINE
Volume 19, Issue 7, Pages 596-602Publisher
ELSEVIER GMBH
DOI: 10.1016/j.phymed.2012.02.010
Keywords
Pannarin; Lichen secondary metabolites; MRSA; Checkerboard assay; Antimicrobial activity; Membrane permeability
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Funding
- MIUR (Ministero dell'Istruzione, dell'Universita e delta Ricerca) EX MURST
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The in vitro antimicrobial activities of pannarin, a depsidone isolated from lichens, collected in several Southern regions of Chile (including Antarctica), was evaluated alone and in combination with five therapeutically available antibiotics, using checkerboard microdilution assay against methicillin-resistant clinical isolates strains of Staphylococcus aureus. MIC90. MIC50, as well as MBC90 and MBC50, were evaluated. A moderate synergistic action was observed in combination with gentamicin, whilst antagonism was observed in combination with levofloxacin. All combinations with erythromycin were indifferent, whilst variability was observed for clindamycin and oxacillin combinations. Data from checkerboard assay were analysed and interpreted using the fractional inhibitory concentration index and the response surface approach using the Delta E model. Discrepancies were found between both methods for some combinations. In order to asses cellular lysis after exposure to pannarin, cell membrane permeability assay was performed. The treatment with pannarin produces bactericidal activity without significant calcein release, consistent with lack of lysis or even significant structural damage to the cytoplasmic membrane. Furthermore, pannarin shows low hemolytic activity and moderate cytotoxic effect on peripheral blood mononuclear cells. These findings suggest that the natural compound pannarin might be a good candidate for the individualization of novel templates for the development of new antimicrobial agents or combinations of drugs for chemotherapy. (C) 2012 Elsevier GmbH. All rights reserved.
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