Journal
PHYTOMEDICINE
Volume 17, Issue 6, Pages 404-409Publisher
ELSEVIER GMBH, URBAN & FISCHER VERLAG
DOI: 10.1016/j.phymed.2010.01.014
Keywords
Spinosin; Semen Ziziphi spinosae; Pentobarbital-induced sleep; 8-OH-DPAT; p-MPPI; 5-HT1A receptor
Categories
Funding
- National Natural Science Foundation of China (NSFC) [30640070, 30772556]
- Janssen Research Council (JRCC)
- Peking University
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Previous results have suggested that spinosin, a C-glycoside flavonoid of Semen Ziziphi spinosae, potentiates pentobarbital-Induced sleep via the serotonergic system. The present study investigated whether spinosin potentiates pentobarbital-induced sleep via serotonin-1A (5-hydroxytryptamine, 5-HT1A) receptors The results demonstrated that spinosin significantly augmented pentobarbital (35 mg/kg, i p)-induced sleep in rats, reflected by reduced sleep latency and increased total sleep time, non-rapid eye movement (NREM) sleep time, and REM sleep time With regard to NREM sleep duration, spinosin mainly increased slow-wave sleep (SWS). Additionally, spinosin (15 mg/kg, i g.) significantly antagonized 5-HT1A agonist 8-OH-DPAT (0.1 mg/kg, i.p.)-induced reductions in total sleep time, NREM sleep, REM sleep, and SWS in pentobarbital-treated rats. These results suggest that spinosin may be an antagonist at postsynaptic 5-HT1A receptors because these effects of 8-OH-DPAT were considered to be mediated via postsynaptic 5-HT1A receptors. Moreover, co-administration of spinosin and the 5-HT1A antagonist 4-iodo-N-{2-[4-(methoxyphenyl)-1-piperazinyl]ethyl}-N-2-pyridinylbenzamide (p-MPPI), at doses that are ineffective when administered alone (spinosin 5 mg/kg, p-MPPI 1 mg/kg), had significant augmentative effects on pentobarbital-induced sleep, reflected by reduced sleep latency and increased total sleep time, NREM sleep, and REM sleep In contrast to the attenuating effects of p-MPPI on REM sleep via presynaptic 5-HT1A autoreceptors, 15 mg/kg spinosin significantly increased REM sleep These results suggest that the effect of spinosin on REM sleep in pentobarbital-treated rats may be related to postsynaptic 5-HT1A receptors (C) 2010 Elsevier GmbH All rights reserved
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