Journal
PHYTOMEDICINE
Volume 16, Issue 6-7, Pages 586-593Publisher
ELSEVIER GMBH, URBAN & FISCHER VERLAG
DOI: 10.1016/j.phymed.2008.12.024
Keywords
Antitumor; Apoptosis; Astragalus mongholicus; Lectin; Cell cycle arrest; Cell proliferation
Categories
Funding
- National Natural Science Foundation of China [20502032]
- Beijing Natural Science Foundation [2063029]
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A lectin (AM M L) from the roots of Astragalus mongholicus was extracted and purified by affinity chromatographic technique. Human cervical carcinoma cell line (HeLa), human osteoblast-like cell line (MG63) and human leukemia cell line (K562) were used to check the effects of AMML on cell proliferation, apoptosis and cell cycle. Maximum growth inhibition (92%) was observed with HeLa cells, followed by K562 cells (84%) and MG63 (48%) cells. Morphological observation showed that AMML-treated HeLa cells displayed outstanding apoptosis characteristics, such as nuclear fragmentation and appearance of membrane-enclosed apoptotic bodies. The apoptosis of HeLa cells was confirmed by flow cytometry using Annexin V/FITC and propidium iodide (PI) staining technique. For the first time we also report a significant cell cycle arrest at S phase of HeLa cells by AMML. Therefore, the present investigation may lead to the possible therapeutic use of Astragalus mongholicus lectin. (C) 2009 Elsevier GmbH. All rights reserved.
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