Journal
PHYTOCHEMISTRY
Volume 145, Issue -, Pages 57-67Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.phytochem.2017.10.005
Keywords
Lichen-associated bacteria; Lichina pygmaea; Alphaproteobacteria; Dereplication workflow; Molecular networking; LC/MSn
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Funding
- INSA Rennes
- project MALICA of the Agence Nationale de la Recherche, France [10-INBS-02-01]
- EMR
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Alphaproteobacterium strain MOLA1416, related to Mycoplana ramosa DSM 7292 and Chelativorans intermedius CC-MHSW-5 (93.6% 16S rRNA sequence identity) was isolated from the marine lichen, Lichina pygmaea and its chemical composition was characterized by a metabolomic network analysis using LC-MS/MS data. Twenty-five putative different compounds were revealed using a dereplication workflow based on MS/MS signatures available through GNPS (https://gnps.ucsd.edu/). In total, ten chemical families were highlighted including isocoumarins, macrolactones, erythrinan alkaloids, prodiginines, isoflavones, cyclohexane-diones, sterols, diketopiperazines, amino-acids and most likely glucocorticoids. Among those compounds, two known metabolites (13 and 26) were isolated and structurally identified and metabolite 26 showed a high cytotoxic activity against B16 melanoma cell lines with an IC50 0.6 +/- 0.07 mu g/mL. (C) 2017 Elsevier Ltd. All rights reserved.
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