Journal
PHYTOCHEMISTRY
Volume 148, Issue -, Pages 122-131Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.phytochem.2017.12.018
Keywords
Cucumis melo L.; Cucurbitaceae; Melon; L-phenylalanine metabolism; Phenylpropanoid aroma volatiles; (E)-cinnamic acid:coenzyme A ligase; Benzoic acid:S-adenosyl-L-methionine carboxyl methyltransferase; (E)-cinnamaldehyde; Methyl benzoate
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Funding
- BARD [IS-4223-09C]
- United States-Israel Binational Agricultural Research and Development Fund
- NSF-BSF Joint Funding Program ICOB
- Ministry of Agriculture of Israel [261-1049-14]
- Agricultural Research Organization, The Volcani Center, Rishon LeZion, Israel [132017]
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Studies on the active pathways and the genes involved in the biosynthesis of L-phenylalanine-derived volatiles in fleshy fruits are sparse. Melon fruit rinds converted stable-isotope labeled L-phe into more than 20 volatiles. Phenylpropanes, phenylpropenes and benzenoids are apparently produced via the well-known phenylpropanoid pathway involving phenylalanine ammonia lyase (PAL) and being (E)-cinnamic acid a key intermediate. Phenethyl derivatives seemed to be derived from L-phe via a separate biosynthetic route not involving (E)-cinnamic acid and PAL. To explore for a biosynthetic route to (E)-cinnamaldehyde in melon rinds, soluble protein cell-free extracts were assayed with (E)-cinnamicacid, CoA, ATP, NADPH and MgSO4, producing (E)-cinnamaldehyde in vitro. In this context, we characterized CmCNL, a gene encoding for (E)-cinnamic acid:coenzyme A ligase, inferred to be involved in the biosynthesis of (E)-cinnamaldehyde. Additionally we describe CmBAMT, a SABATH gene family member encoding a benzoic acid:S-adenosyl-L-methionine carboxyl methyltransferase having a role in the accumulation of methyl benzoate. Our approach leads to a more comprehensive understanding of L-phe metabolism into aromatic volatiles in melon fruit. (C) 2018 Elsevier Ltd. All rights reserved.
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