Journal
PHYTOCHEMISTRY
Volume 95, Issue -, Pages 118-126Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.phytochem.2013.06.025
Keywords
Wheat; Triticum aestivum L. cv. Yangmai 3E-158; Subtilisin-like serine protease; EP3 protease; Leaf senescence
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Funding
- National Natural Science Foundation of China [31000363]
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Senescence-related proteases play important roles in leaf senescence by regulating protein degradation and nutrient recycling. A 98.9 kDa senescence-related protease EP3 in wheat leaves was purified by ammonium sulfate precipitation, Q-Sepharose fast flow anion exchange chromatography and gel slicing after gel electrophoresis. Due to its relatively high thermal stability, its protease activity did not decrease after incubation at 40 degrees C for 1-h. EP3 protease was suggested to be a metal-dependent serine protease, because its activity was inhibited by serine protease inhibitors PMSF and AEBSF and metal related protease inhibitor EGTA. It was identified as a subtilisin-like serine protease of the S8A family based on data from both mass spectrometry and the cloned cDNA sequence. Therefore, these data suggest that a serine protease of the S8A subfamily with specific biochemical properties is involved in senescence-associated protein degradation. (C) 2013 Elsevier Ltd. All rights reserved.
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