Journal
PHYTOCHEMISTRY
Volume 95, Issue -, Pages 259-267Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.phytochem.2013.07.023
Keywords
Sophora flavescens; Leguminosae; Asthma; Traditional Chinese herbal medicine; ASHMI; Ku Shen; Trifolirhizin; Airway smooth muscle contraction
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Funding
- NIH/NCCAM, Center for Chinese Herbal Therapy (CHT) for Asthma [1P01 AT002644725-01]
- Utah State University faculty startup fund
- NIH/NCCAM Ruth L. Kirchstein Pre-doctoral fellowship award [F31AT003769]
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Asthma is a serious health problem worldwide, particularly in industrialized countries. Despite a better understanding of the pathophysiology of asthma, there are still considerable gaps in knowledge as well as a need for classes of drugs. ASHMI (TM) (Anti-asthma Herbal Medicine Intervention) is an aqueous extract of Ganoderma lucidum (Fr.) P. Karst (Ling Zhi), Sophora flavescens Aiton (Ku Shen) and Glycyrrhiza uralensis Fisch. ex DC (Gan Cao). It prevents allergic asthma airway hyper-reactivity in mice and inhibits acetylcholine (ACh) induced airway smooth muscle (ASM) contraction in tracheal rings from allergic asthmatic mice. The purpose of this research was to identify individual herb(s) and their active compound(s) that inhibit ASM contraction. It was found that S. flavescens, but not G. lucidum or G. uralensis aqueous extracts, inhibited ASM contraction in tracheal rings from asthmatic mice. Bioassay-guided isolation and identification of flavonoid fractions/compound(s) via methylene chloride extraction, preparative HPLC fractionation, and LC-MS and NMR spectroscopic analyses showed that trifolirhizin is an active constituent that inhibits acetylcholine mediated ASM contraction or directly relaxes pre-contracted ASM independent of beta(2)-adrenoceptors. (C) 2013 Elsevier Ltd. All rights reserved.
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