Journal
PHYTOCHEMISTRY
Volume 73, Issue -, Pages 114-118Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.phytochem.2011.09.013
Keywords
Arabidopsis thaliana; Flow injection electrospray mass spectrometry; Flavonol glycosides; Cruciferae; Root tips
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Funding
- NSF [IOB-0820674]
- Virginia Tech Mass Spectrometry Incubator by the Fralin Life Science Institute
- Division Of Integrative Organismal Systems
- Direct For Biological Sciences [0820674] Funding Source: National Science Foundation
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Developments in mass spectrometry-based technologies are offering insights into the complexity and dynamic nature of plant metabolism. However, the ability to generate reliable metabolic profiles at high spatial resolution is still limited by the need of most technologies for large sample sizes or time-intensive extraction and detection methods. Here we describe the use of flow injection electrospray mass spectrometry for the rapid identification and semi-quantitative analysis of flavonol glycosides in individual root tips. This method uncovered spatial and temporal differences in metabolic profiles that were masked in analyses of whole roots or seedlings, while showing that individual biological replicates can be extremely consistent. (C) 2011 Elsevier Ltd. All rights reserved.
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