Journal
PHYTOCHEMISTRY
Volume 70, Issue 3, Pages 414-418Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.phytochem.2008.12.018
Keywords
Rhaponticum carthamoides; Essential oil; 13-Norcypera-1(5), 11(12)-diene; Aplotaxene; Cyperene; Antimicrobial activity
Categories
Funding
- Ministry of Education, Youth and Sports [MSM 60460701]
- Academy of Sciences of the Czech Republic [Z40550506]
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A detailed analysis of Rhaponticum carthamoides (Willd.) Iljin root essential oil was carried out by GC, GC-MS and GC-FTIR techniques. In total, 30 components were identified, accounting for 98.0% of total volatiles. A norsesquiterpene 13-norcypera-1(5), 11(12)-diene (22.6%), followed by aplotaxene (21.2%) and cyperene (17.9%), were isolated and their structures confirmed by 1D and 2D-NMR spectra (COSY, ROESY, HSQC, HMBC and INADEQUATE). Selinene type sesquiterpenes and aliphatic hydrocarbons were among minor constituents of the essential oil. The oil exhibited antimicrobial activity against 5 of 9 strains of bacteria and yeast, when tested using broth micro-dilution method. Minimum inhibitory concentrations ranged between 32 and 256 mu g/ml. (C) 2009 Elsevier Ltd. All rights reserved.
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