Anti-herpetic activity of a sulfated xylomannan from Scinaia hatei

Many viruses display affinity for cell surface heparan sulfate proteoglycans with biological relevance in virus entry. This raises the possibility of the application of sulfated polysaccharides in antiviral therapy. In this study we have analyzed polysaccharide fractions isolated from Scinaia hatei. The crude water extract (ShWE) as well as one fraction (F1) obtained by size exclusion chromatography had potent anti-HSV activity. Their inhibitory concentration 50% (IC50) values ranging from 0.5 to 4.6 mu g/ml were Much lower than the cytotoxic concentration 50% (CC50) values (>= 1000 mu g/ml). These fractions had very low anticoagulant activity. Furthermore, they had a weak inactivating effect on virions in a virucidal assay at concentrations in the range of 60-100 mu g/ml. Chemical, chromatographic and spectroscopic methods showed that the major polysaccharide, which had 0.4 sulfate group per monomer unit and an apparent molecular mass of 160 kDa, contained a backbone of alpha-(1 -> 3)-linked D-mannopyranosyl residues substituted at C-6, C-4 and C-2 with single stub Of P-D-Xylopyranosyl residues. Sulfate groups, when present, are located at C-4 of alpha-(1 -> 3)-linked D-mannopyranosyl units, and appeared to be very important for the anti-herpetic activity of this polymer. (C) 2008 Elsevier Ltd. All rights reserved.