Journal
PHYTOCHEMISTRY
Volume 69, Issue 16, Pages 2849-2855Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.phytochem.2008.08.021
Keywords
Amphidinium carterae; Marine dinoflagellate; Odd very-long-chain polyunsaturated fatty acids; Liquid chromatography-mass spectrometry-atmospheric pressure chemical ionization
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Funding
- Institutional Research Concepts [AV0Z50200510, AV0Z60050516, MSMT 002162082]
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A method is described for the enrichment of odd very-long-chain polyunsaturated fatty acids (VLCPUFAs) by means of RP-HPLC and argentation TLC from total fatty acids of the dinoflagellate A. carterae and their identification as picolinyl esters by means of microbore liquid chromatography-mass spectrometry with atmospheric pressure chemical ionization (LC-MS/APCI). The combination of argentation TLC and LC-MS/ APCI was used to identify rare and unusual odd VLCPUFAs up to nonacosahexaenoic acid. Two acids, (allZ)-nonacosa-11,14,17,20,23-pentaenoic acid (29:5n-6) and (allZ)-nonacosa-11,14,17,20,23,26-hexaenoic acid (29:6n-3), were synthesized for the first time to unambiguously confirm their structure. Possible biosynthetic pathways for odd VLCPUFAs are also proposed. All rights reserved (C) 2008 Elsevier Ltd..
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