Journal
PHYTOCHEMISTRY
Volume 69, Issue 2, Pages 479-490Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.phytochem.2007.06.025
Keywords
Chlamydomonas nivalis; snow alga; astaxanthin diglucoside diesters; liquid chromatography-mass spectrometry-atmospheric pressure chemical ionization
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A method is described for the identification of astaxanthin glucoside esters from snow alga Chlamydomonas nivalis by means of liquid chromatography-mass spectrometry with atmospheric pressure chemical ionization (LC-MS/APCI). The method is based on the use of preparative HPLC and subsequent identification of astaxanthin diglucoside diesters by microbore LC-MS/APCI. The combination of these two techniques was used to identify more than 100 molecular species. The astaxanthin diglucoside diester, i.e. (all-E)-[di-(6-O-oleoyl-beta-D-glucopyranosyloxy)]-astaxanthin, was also synthesized to unambiguously confirm its structure. (C) 2007 Elsevier Ltd. All rights reserved.
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