Journal
PHYTOCHEMISTRY
Volume 69, Issue 16, Pages 2799-2806Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.phytochem.2008.08.019
Keywords
Dactylis glomerata; Orchard grass; Polyphenol oxidase; Hydroxycinnamate esters; Isocitric acid; Hydroxycitric acid; Threonic acid
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Funding
- Biotechnology and Biological Sciences Research Council [BBS/E/W/00003134D] Funding Source: Medline
- Biotechnology and Biological Sciences Research Council [BBS/E/W/00003134D] Funding Source: researchfish
- BBSRC [BBS/E/W/00003134D] Funding Source: UKRI
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Polyphenol oxidase (PPO) activity has been reported in orchard grass (Dactylis glomerata); however, to date, no endogenous substrates have been identified. In the present study, we report the isolation and structural elucidation of PPO substrates in this species. The free phenol fraction was extracted, separated by reverse-phase chromatography and six potential substrates, including two hydroxycinnamate esters, were identified by UV spectrometry, electrospray ionisation-tandem mass spectrometry (LC-ESI-MSn) and 1D and 2D NMR analyses (H-1 NMR, C-13 NMR, DEPT, COSY, HMQC and HMBC). Furthermore, three caffeoylquinic acids (3-CQA, 4-CQA and 5-CQA) were identified by comparison of their spectral data (ESI-MS) with those of known compounds and literature data. Five of these compounds were demonstrated to be substrates for orchard grass PPO. (C) 2008 Elsevier Ltd. All rights reserved.
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