Journal
PHYTOCHEMICAL ANALYSIS
Volume 23, Issue 6, Pages 668-676Publisher
WILEY
DOI: 10.1002/pca.2371
Keywords
UHPLC; ESI-QTOF-MS; MS; phenylethanoid glycosides; Cistanches deserticola Y; C; Ma
Funding
- Program for Changjiang Scholars and Innovative Research Team in University [PCSIRT IRT0973]
- Project of International Cooperation Plan from the Ministry of Science and Technology of China [2008DFB30070]
- Tianjin Municipal Higher School Science and Technology Development Fund Program [20090201]
- Scientific Program of Left Banner of Alashan [2011-19]
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Introduction Phenylethanoid glycosides (PhGs) are the major active constituents of Cistanches deserticola Y.C. Ma. However, the isolation, purification and identification procedures of PhGs are difficult and time-consuming. Objective To establish a rapid and sensitive ultrahigh-pressure liquid chromatography (UHPLC)/ESI (electrospray ion source)quadrupole time of flight (QTOF)MS/MS method that could be applied to rapidly profile and identify PhGs. Methodology Seven standard compounds were used for the investigation of the fragmentation pattern. Based on the UHPLC/ESIQTOFMS/MS method, the important structural information on the types of aglycone and saccharide sequences present should be obtained. Results According to the HPLC retention behaviour, the proposed fragmentation pathways provided by high-resolution MS and MS/MS spectra and literature sources, a total of 13 PhGs in the crude extract of C. deserticola were identified or tentatively identified. Conclusion A rapid and accurate UHPLC/ESIQTOFMS/MS method was established for the identification of PhGs in the crude extract of C. deserticola. This method therefore can be used for rapid prediction of the chemical constituents and qualities of this plant. Copyright (c) 2012 John Wiley & Sons, Ltd.
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