Journal
PHYSIOLOGICAL GENOMICS
Volume 32, Issue 2, Pages 207-218Publisher
AMER PHYSIOLOGICAL SOC
DOI: 10.1152/physiolgenomics.00017.2007
Keywords
atrophy; axotomy; denervation; neuromuscular disease
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Muscle atrophy is a major hallmark of amyotrophic lateral sclerosis (ALS), the most frequent adult-onset motor neuron disease. To define the full set of alterations in gene expression in skeletal muscle during the course of the disease, we used the G86R superoxide dismutase-1 transgenic mouse model of ALS and performed high-density oligonucleotide microarrays. We compared these data to those obtained by axotomy-induced denervation. A major set of gene regulations in G86R muscles resembled those of surgically denervated muscles, but many others appeared specific to the ALS condition. The first significant transcriptional changes appeared in a subpopulation of mice before the onset of overt clinical symptoms and motor neuron death. These early changes affected genes involved in detoxification (e. g., ALDH3, metallothionein-2, and thioredoxin-1) and regeneration (e. g., BTG1, RB1, and RUNX1) but also tissue degradation (e. g., C/EBP delta and DDIT4) and cell death (e. g., ankyrin repeat domain-1, CDKN1A, GADD45 alpha, and PEG3). Of particular interest, metallothionein-1 and -2, ATF3, cathepsin-Z, and galectin-3 genes appeared, among others, commonly regulated in both skeletal muscle (our present data) and spinal motor neurons (as previously reported) of paralyzed ALS mice. The importance of these findings is twofold. First, they designate the distal part of the motor unit as a primary site of disease. Second, they identify specific gene regulations to be explored in the search for therapeutic strategies that could alleviate disease before motor neuron death manifests clinically.
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