Journal
PHYSIOLOGICAL ENTOMOLOGY
Volume 33, Issue 4, Pages 365-371Publisher
WILEY
DOI: 10.1111/j.1365-3032.2008.00642.x
Keywords
Ostrinia nubilalis; age-dependent; cold hardiness; diapause; glycerol; lethal temperatures; supercooling point; trehalose
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The age-dependent cold hardiness profile of Ostrinia nubilalis is compared between nondiapausing and diapausing larvae, as well as with field-collected larvae. The results suggest that both cold tolerance and accumulation of cryoprotectants depends upon the age of O. nubilalis larva. Late fifth-instar nondiapausing larvae are more cold tolerant than younger fifth-instars because they show enhanced ability to withstand sub-zero temperatures. No appreciable difference is observed between the experimental groups of diapausing larvae as far as their supercooling ability and tolerance at sub-zero temperatures above the supercooling point. In general, both field-collected and diapausing larvae are more cold tolerant than nondiapausing larvae, indicating a direct link between diapause and cold hardiness. The age of diapausing larvae affects the ability to accumulate glycerol. Glycerol levels of 45-day-old diapausing larvae are significantly higher (2.7-fold) compared with 90-day-old diapausing larvae. Moreover, diapausing larvae display a five- to 13-fold higher glycerol content compared with nondiapausing larvae. There is a trend for an age-dependent cold hardiness profile in O. nubilalis and further tests that could demonstrate a causal relationship between age and cold tolerance are needed.
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