Journal
VIROLOGY
Volume 483, Issue -, Pages 229-235Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.virol.2015.04.020
Keywords
Reovirus; Interferon; Reverse genetics; RNA capping; RNA methyltransferase
Categories
Funding
- Natural Sciences and Engineering Research Council of Canada (NSERC) [155580]
- Faculte des etudes superieures of Universite de Montreal
Ask authors/readers for more resources
In the last few years, the development of a plasmid-based reverse genetics system for mammalian reovirus has allowed the production and characterization of mutant viruses. This could be especially significant in the optimization of reovirus strains for virotherapeutic applications, either as gene vectors or oncolytic viruses. The genome of a mutant virus exhibiting increased sensitivity to interferon was completely sequenced and compared with its parental virus. Viruses corresponding to either the parental or mutant viruses were then rescued by reverse genetics and shown to exhibit the expected phenotypes. Systematic rescue of different viruses harboring either of the four parental genes in a mutant virus backbone, or reciprocally, indicated that a single amino acid substitution in one of lambda 2 methyltransferase domains is the major determinant of the difference in interferon sensitivity between these two viruses. (C) 2015 Elsevier Inc. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available