Journal
VIRAL IMMUNOLOGY
Volume 28, Issue 9, Pages 489-494Publisher
MARY ANN LIEBERT, INC
DOI: 10.1089/vim.2015.0030
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Funding
- Key Program for International S&T Cooperation Projects of Shaanxi Province [2012KW-20]
- Key Construction Program of International Cooperation Base in S&T Shaanxi Province [2015SD0018]
- Ministry of Education
- State Administration of Foreign Experts Affairs Overseas Teacher project, China [MS2011XBNL057]
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Immunoglobulin Y (IgY) antibodies were generated against canine parvovirus virus-like particles (CPV-VLPs) antigen using chickens. Anti-CPV-VLPs-IgY was extracted from hen egg yolk and used for developing enzyme-linked immunosorbent assay (ELISA) and immunochromatographic assay (ICA) for the detection of CPV in dog feces. The cutoff negative values for anti-CPV-VLPs-IgY were determined using negative fecal samples (already confirmed by polymerase chain reaction [PCR]). In both ELISA and ICA, there was no cross-reaction with other diarrheal pathogens. Thirty-four fecal samples were collected from dogs with diarrhea, of which 26.47% were confirmed as CPV-positive samples by PCR, while 29.41% and 32.35% of the samples were found to be positive by ELISA and ICA, respectively. The developed ELISA and ICA exhibited 97.06% and 94.12% conformity with PCR. Higher sensitivity and specificity were observed for IgY-based ELISA and ICA. Thus, they could be suitable for routine use in the diagnosis of CPV in dogs.
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