Journal
PHYSICAL REVIEW LETTERS
Volume 106, Issue 4, Pages -Publisher
AMER PHYSICAL SOC
DOI: 10.1103/PhysRevLett.106.048104
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Funding
- U.S.-Israel Binational Science Foundation
- Israel Science Foundation
- Minerva Foundation
- National Science Foundation [PHY-0750133]
- Division Of Physics
- Direct For Mathematical & Physical Scien [0750133] Funding Source: National Science Foundation
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A complete gene expression reaction is reconstituted in a cell-free system comprising the entire endogenous transcription, translation, as well as mRNA and protein degradation machinery of E. coli. In dissecting the major reaction steps, we derive a coarse-grained enzymatic description of biosynthesis and degradation, from which ten relevant rate constants and concentrations are determined. Governed by zeroth-order degradation, protein expression follows a sharp transition from undetectable levels to constant-rate accumulation, without reaching steady state.
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