Journal
VETERINARY MICROBIOLOGY
Volume 177, Issue 3-4, Pages 379-385Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.vetmic.2015.03.023
Keywords
Pseudorabies virus; US3; Actin; Entry
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Funding
- Agency for Innovation by Science and Technology in Flanders (IWT-Vlaanderen)
- F.W.O.-Vlaanderen [G.0835.09]
- Research Council of Ghent University [01G01311]
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The conserved alphaherpesvirus US3 tegument protein induces rearrangements of the actin cytoskeleton, consisting of protrusion formation and stress fiber breakdown. Although US3 does not affect levels of total actin protein, it remains unclear whether US3 modulates the total levels of filamentous (F) actin. In this report, we show that the pseudorabies virus (PRV) US3 protein, via its kinase activity, leads to disassembly of F-actin in porcine ST cells. F-actin disassembly has been reported before to contribute to host cell entry of HIV. In line with this, in the current study, we report that US3 has a previously uncharacterized role in viral genome delivery to the nucleus, since quantitative polymerase chain reaction (qPCR) assays on nuclear fractions demonstrated a reduced nuclear delivery of US3null PRV compared to wild type PRV genomes. Treatment of cells with the actin depolymerizing drug cytochalasin D enhanced virus genome delivery to the nucleus, particularly of US3null PRV, supporting a role for F-actin disassembly during certain aspects of viral entry. In conclusion, the US3 kinase of PRV leads to F-actin depolymerization, and US3 and F-actin disassembly contribute to viral genome delivery to the nucleus. (C) 2015 Elsevier B.V. All rights reserved.
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