4.7 Article

Influenza A(H1N1)pdm09 virus in pigs, Togo, 2013

Journal

VETERINARY MICROBIOLOGY
Volume 177, Issue 1-2, Pages 201-205

Publisher

ELSEVIER SCIENCE BV
DOI: 10.1016/j.vetmic.2015.02.028

Keywords

Influenza virus; H1N1; Pandemic; Togo; Swine; Interspecies transmission

Funding

  1. U.S. National Institute of Allergy and Infectious Diseases, National Institutes of Health Department of Health and Human Services [HHSN266200700005C]
  2. American Lebanese Syrian Associated Charities (ALSAC)

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We collected 325 nasal swabs from freshly slaughtered previously healthy pigs from October 2012 through January 2014 in a slaughterhouse near Lome in Togo. Influenza A virus genome was detected by RT-PCR in 2.5-12.3% of the pooled samples, and results of hemagglutinin subtyping RT-PCR assays showed the virus in all the positive pools to be A(H1N1)pdm09. Virus was isolated on MDCK cells from a representative specimen, A/swine/Togo/ONA32/2013(H1N1). The isolate was fully sequenced and harbored eight genes similar to A(H1N1)pdm09 virus genes circulating in humans in 2012-2013, suggesting human-to-swine transmission of the pathogen. (C) 2015 Elsevier B.V. All rights reserved.

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