Journal
VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY
Volume 167, Issue 1-2, Pages 64-69Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.vetimm.2015.06.006
Keywords
Fowl paratyphoid; Macrophage; Cytokine; Vaccine; Adjuvant; TLR2
Categories
Funding
- FAPESP [2012/24017-7, 2013/26127-7]
- CAPES
- FUNDUNESP
Ask authors/readers for more resources
Lactobacillus-based probiotics (LBP) are used as competitive exclusion to control pathogenic enterobacterial infections and improve the weight gain in broiler chickens. This study assessed the inhibition of Salmonella Enteritidis (SE) infection in one-week-old broiler chicks, using an experimental LBP containing four Lactobacillus strains isolated from chickens (L acidophilus, L. fermentum, L. reuteri, L. salivarius). The immunomodulatory effects of this treatment were evaluated, through the analysis of cytokines and influx of macrophages, gamma delta, CD4(+) and CD8(+) T cells in the gut. The intestinal colonization by SE was reduced by 1.8 CFU/g (log(10)) in chicks treated with LBP (p < 0.05). The levels of pro-inflammatory cytokines (IL-1 beta, LITAF) were significantly reduced in treated chicks (p < 0.05), whilst untreated chicks showed elevated inflammatory stimulus and an increased population of CD8(+) T cells in the intestinal mucosa after challenge (p < 0.05). Additionally, the LBP stimulated TLR2 expression in caecal tonsils. The adjuvant property of the Lactobacillus cell wall (LCW) was evaluated, demonstrating good capability to stimulate T helper 2 (Th2) cell proliferation. Pretreatment of chicks with LBP decreased the intestinal colonization by SE, minimizing the tissue lesions and inflammation after challenge and showed a potential use as adjuvant with injectable killed vaccines. (C) 2015 Elsevier B.V. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available