Journal
PHYSICAL REVIEW B
Volume 84, Issue 21, Pages -Publisher
AMER PHYSICAL SOC
DOI: 10.1103/PhysRevB.84.214111
Keywords
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Funding
- Max Planck Society
- Helmholtz Association
- CBST at UC Davis
- DOE [DE-SC0002141]
- National Science foundation NSF [0417142, MCB-1021557]
- National Institute of Health [1R01GM09558301, 1U54GM094625-01]
- U.S. Department of Energy through the PULSE Institute at the SLAC National Accelerator Laboratory
- Lawrence Livermore National Laboratory [DE-AC52-07NA27344]
- Joachim Herz Stiftung
- Div Of Molecular and Cellular Bioscience
- Direct For Biological Sciences [1120997] Funding Source: National Science Foundation
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X-ray free-electron lasers deliver intense femtosecond pulses that promise to yield high resolution diffraction data of nanocrystals before the destruction of the sample by radiation damage. Diffraction intensities of lysozyme nanocrystals collected at the Linac Coherent Light Source using 2 keV photons were used for structure determination by molecular replacement and analyzed for radiation damage as a function of pulse length and fluence. Signatures of radiation damage are observed for pulses as short as 70 fs. Parametric scaling used in conventional crystallography does not account for the observed effects.
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