Journal
ARCHIVES OF VIROLOGY
Volume 160, Issue 5, Pages 1211-1217Publisher
SPRINGER WIEN
DOI: 10.1007/s00705-015-2382-6
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Funding
- National Science and Technology Support Program [2012BAD19B03]
- China 973 Program [2010CB126203]
- Special Fund for Agro-Scientific Research in the Public Interest of China [201003031]
- 863 program [2007AA10Z414]
- Zhejiang Provincial Science and Technology Project [2010C12027]
- Zhejiang Provincial Foundation for Natural Science [Z305165, Y3090657]
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The genome segment S5 of rice black-streaked dwarf virus (genus Fijivirus, family Reoviridae) is functionally bicistronic in infected plants. It has a conserved second ORF (P5-2) partially overlapping the major ORF in a different reading frame, but its function remains unknown. P5-2 was detected in infected plants, but not in purified viral particles by Western blotting, indicating that it is a non-structural protein. In immunoelectron microscopy, polyclonal antibodies against P5-2 specifically labelled chloroplasts of infected rice plants. When P5-2 fused with green fluorescent protein was transiently expressed in leaves of Nicotiana benthamiana, fluorescence was also co-localized with chloroplasts. Experiments with deletion mutants of P5-2 showed that its N-terminal part was responsible for its targeting to chloroplasts.
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