Journal
PHYSICAL CHEMISTRY CHEMICAL PHYSICS
Volume 20, Issue 43, Pages 27245-27255Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c8cp02942c
Keywords
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Funding
- Ministry of Science and Technology in Taiwan [MOST 105-2112-M-008-007-MY3]
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Super-resolution imaging based on single-molecule localization microscopy combined with the surface plasmon polariton (SPP)-enhanced fluorescence of spontaneously blinking fluorophores was demonstrated to visualize the nanoscale-level positioning information of cell-adhesion-associated proteins. Glass substrates with a deposited silver layer were utilized to induce a SPP-enhanced field on the silver surface and significantly strengthen the fluorescence signals of the fluorophores by more than 300%. The illumination power density for localization imaging at a spatial resolution of 25 +/- 11 nm was 31.6 W cm (2). This low illumination power density will facilitate the reduction of phototoxicity of the biospecimens for single-molecule localization imaging. The proposed strategy provides a uniform distribution of the SPP-enhanced field on the silver surface, enabling visualization of the spatial distribution of labeled proteins without interference caused by the enhanced field distribution.
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