Journal
PHYSICAL CHEMISTRY CHEMICAL PHYSICS
Volume 15, Issue 16, Pages 6008-6015Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c3cp00076a
Keywords
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Funding
- National Institutes of Health of U.S.A. [R01 EB006201, R01 ES014774]
- U.S. Defense Advanced Research Projects Agency [DARPA-N66001-09-C-2082]
- Nanoscale Research Facility (NRF) at the Indian Institute of Technology-Delhi, India
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The development of DNA detection techniques on large-area plasmonics-active platforms is critical for many medical applications such as high-throughput screening, medical diagnosis and systems biology research. Here, we report for the first time a unique molecular sentinel-on-chip'' (MSC) technology for surface-enhanced Raman scattering (SERS)-based DNA detection. This unique approach allows label-free detection of DNA molecules on chips developed on a wafer scale using large area nanofabrication methodologies. To develop plasmonics-active biosensing platforms in a repeatable and reproducible manner, we employed a combination of deep UV lithography, atomic layer deposition, and metal deposition to fabricate triangular-shaped nanowire (TSNW) arrays having controlled sub-10 nm gap nanostructures over an entire 6 inch wafer. The detection of a DNA sequence of the Ki-67 gene, a critical breast cancer biomarker, on the TSNW substrate illustrates the usefulness and potential of the MSC technology as a novel SERS-based DNA detection method.
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