Journal
ARCHIVES OF VIROLOGY
Volume 160, Issue 12, Pages 3075-3078Publisher
SPRINGER WIEN
DOI: 10.1007/s00705-015-2586-9
Keywords
Detection; RT-LAMP; TBRV
Categories
Funding
- National Science Centre [2013/11/B/NZ9/02510]
- Ministry of Science and Higher Education [IP2014 014973]
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A reverse transcription loop-mediated isothermal amplification assay (RT-LAMP) has been developed for detection of tomato black ring virus (TBRV) isolates collected from different hosts. One-step RT-LAMP was performed with a set of four primers, the design of which was based on the coat protein gene. Results of RT-LAMP were visualized by direct staining of products with fluorescent dyes, agarose gel electrophoresis, and analysis of amplification curves. The sensitivity of RT-LAMP was 100-fold greater than that of RT-PCR. The RT-LAMP assay developed here is a useful and practical method for diagnosis of TBRV.
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