Journal
ARCHIVES OF VIROLOGY
Volume 160, Issue 7, Pages 1775-1779Publisher
SPRINGER WIEN
DOI: 10.1007/s00705-015-2435-x
Keywords
Cosavirus; qPCR; Wastewater; Nested PCR; Phylogenetic analysis
Categories
Funding
- National Science Foundation (NSF) Water and Environmental Technology (WET) Center, The University of Arizona
- Japan Society for the Promotion of Science (JSPS) under JSPS Postdoctoral Fellowships for Research Abroad [517]
- Fulbright Master of Science and Technology Scholarship
- Directorate For Engineering
- Div Of Industrial Innovation & Partnersh [1361505] Funding Source: National Science Foundation
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Human cosavirus (HCoSV) is a novel member of the family Picornaviridae. We investigated the prevalence and genetic diversity of HCoSV in influent and effluent wastewater in Arizona over a 12-month period, from August 2011 to July 2012. HCoSV sequences were identified in six (25 %) influent samples and one (4 %) effluent sample, with the highest concentration of 3.24 x 10(5) and 1.54 x 10(3) copies/liter in influent and effluent, respectively. The strains were characterized based on their 5' untranslated region and classified into species A and D, demonstrating that genetically heterogeneous HCoSV were circulating with a clear temporal shift of predominant strains in the study area.
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