Journal
PHYSICAL CHEMISTRY CHEMICAL PHYSICS
Volume 13, Issue 23, Pages 11110-11117Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c1cp20354a
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Funding
- DAAD
- Ministry of Education and Science of Russian Federation [02.740.11.5226]
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Enzyme based micron sized sensing system with optical readout was fabricated by co-encapsulation of urease and dextran couple with pH sensitive dye SNARF-1 into polyelectrolyte multilayer capsules. Co-precipitation of calcium carbonate, urease and dextran followed up by multilayer film coating and Ca-extracting by EDTA resulted in the formation of 3.5-4 micron capsules, what enable the calibrated fluorescence response to urea in concentration range from 10(-6) to 10(-1) M. The presence of urea can be monitored on a single capsule level as illustrated by confocal fluorescent microscopy. Variations in urease: dye ratio in capsules, applicability and limits of use of that type multi-component microencapsulated sensors are discussed.
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