Journal
PHYSICAL CHEMISTRY CHEMICAL PHYSICS
Volume 12, Issue 41, Pages 13408-13418Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c0cp00680g
Keywords
-
Funding
- Royal Society
- EPSRC
- BBSRC
- Advantage West Midlands
- European Regional Development Fund
Ask authors/readers for more resources
The direct observation of binding and release of spectroscopically silent metal ions such as Zn2+ and Ca2+ by proteins has been challenging before the advent of native electrospray ionisation mass spectrometry. This report highlights the powerful capability of ESI-MS to provide insight into metalloprotein speciation that is independent of any spectroscopic property. Using the zinc-binding plant metallothionein E-C from wheat as a study case, we show that ESI-MS is unique amongst other techniques in capturing intermediary metallospecies that evolve during the course of metal transfer to the chelator EDTA, as a model reaction to mimic the biological function of the protein as a zinc donor. Zinc release from the two-domain protein E-C appears to be extremely rapid and non-cooperative, and progresses with loss of one zinc ion from the fully loaded Zn-6 species, and a transient build-up of Zn-5 and Zn-4 species, which further react to give species with 0-3 zinc ions bound. H-1 NMR data has provided further insights into the different behaviour of the two domains upon metal depletion.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available