Journal
PHYSICAL CHEMISTRY CHEMICAL PHYSICS
Volume 12, Issue 38, Pages 12153-12159Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c0cp00378f
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Funding
- Center for Advanced Bionanosystems, Nanyang Technological University, Singapore
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A hollow sphere-nanostructured conductive polymer/metal oxide composite was synthesized and used to investigate the electrochemical behavior of glucose oxidase, demonstrating a significantly enhanced direct electron transfer ability of glucose oxidase. In particular, the long-standing puzzle of whether enzymatic glucose sensing involves an enzyme direct electron transfer process was studied. The results indicate the mechanism is indeed a glucose oxidase direct electron transfer process with competitive glucose oxidation and oxygen reduction to detect glucose. A glucose biosensor with the glucose oxidase-immobilized nanomaterial was further constructed, demonstrating superior sensitivity and reliability, and providing great potential in clinical applications.
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