Journal
PHYSICA STATUS SOLIDI B-BASIC SOLID STATE PHYSICS
Volume 246, Issue 11-12, Pages 2832-2835Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/pssb.200982257
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Funding
- GAAV [KAN400100701]
- MSMT [LC06040, LC510]
- Fellowship J.E. Purkyne (ASCR).
- [AV0Z10100521]
- [MSM0021620806]
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We investigate adhesion of fetal bovine serum (FBS) on diamond surfaces which have alternating H/O-terminated surface patterns of 30 mu m width prepared by hydrogen and oxygen plasma. The samples are immersed into 15% FBS in McCoy's 5A supporting medium and characterized by in situ atomic force microscopy (AFM) during 6 days of adsorption. Within the first day, 2-4 nm primary layer is formed on both H/O-terminated surfaces. After 6 days, we observe 17 +/- 5 nm FBS layer on O-terminated surface and 35 +/- 5 nm layer on H-terminated surface. Adhesion of the primary and secondary layer is weaker on H-terminated surface. We present a model of FBS protein layers on H-/O-terminated diamond and we discuss implications for a preferential cell growth on O-terminated diamond. We also show that the preferential cell growth is not affected by the long-term FBS pre-adsorption. (C) 2009 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
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