Journal
PHYSICA STATUS SOLIDI A-APPLICATIONS AND MATERIALS SCIENCE
Volume 208, Issue 6, Pages 1440-1445Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/pssa.201000320
Keywords
biomaterial; electrochemistry; gradient surfaces; porous silicon; surface chemistry
Funding
- CSIRO
- Australian Synchrotron
- Australian Research Council
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Chemical gradients of ethyl-6-bromohexanoate (EBH) were generated over porous silicon (pSi) substrates via electrochemical attachment. Immobilised ester moieties were hydrolysed and activated to produce a gradient of functional carboxylic acid groups. After subsequent immobilisation of cyclic RGD (cRGD), the surfaces were used to screen the extent of rat mesenchymal stem cell (MSC) attachment. Mapping of surface chemistry was carried out by means of infrared microscopy and X-ray photoelectron spectroscopy (XPS). MSC culture studies showed that short-term cell attachment responded to the cRGD density present on the gradient surface. (C) 2011 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
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