Journal
ULTRASONICS SONOCHEMISTRY
Volume 22, Issue -, Pages 243-248Publisher
ELSEVIER
DOI: 10.1016/j.ultsonch.2014.05.006
Keywords
Ultrasound; Cell disruption; Polysaccharide-protein complex; Medicinal fungus; Mycelial broth; Kinetic model
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Funding
- Hong Kong Government UGC (GRF) [PolyU 5036/10P, PolyU 5033/11P]
- Hong Kong Polytechnic University
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High-intensity ultrasound (US) was applied to facilitate the extraction of intracellular and extracellular polysaccharide-protein complexes (PSPs) from the viscous mycelial fermentation broth of a medicinal fungus Cordyceps sinensis Cs-HK1. The US treatment caused the disruption of fungal mycelia, a dramatic reduction of the apparent broth viscosity, and the release of intracellular products into the liquid medium. The degree of mycelium disruption and the rate of intracellular product release were dependent on US power intensity, treatment period and biomass concentration of broth. The extraction or release kinetics of total water-soluble products and PSPs (yield Y versus time t) under the effect of US was fitted closely to the Elovich model Y = Y-0 + Y-1 In t and parabolic model Y = Y-0 + Y(1)t(1/2), respectively. Another interesting effect of the US treatment was a notable increase in the antioxidant cytoprotective activity of PSP against H2O2 induced cell death. (C) 2014 Elsevier B.V. All rights reserved.
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