Journal
PHOTOSYNTHESIS RESEARCH
Volume 120, Issue 1-2, Pages 43-58Publisher
SPRINGER
DOI: 10.1007/s11120-013-9806-5
Keywords
Chl a fluorescence; Fluorescence yield changes; Light-induced conformational changes; OJIP transient; Thermal phase
Categories
Funding
- Hungarian Research Foundation (OTKA) [MB08B82403, PD72718, CNK80345]
- NIH-A*STAR [TET-10-1-2011-0279]
- Bolyai Janos Research Foundation of the Hungarian Academy of Sciences
- Marie Curie Initial Training Network HARVEST
- European Union [238017]
Ask authors/readers for more resources
Chlorophyll a fluorescence is a non-invasive tool widely used in photosynthesis research. According to the dominant interpretation, based on the model proposed by Duysens and Sweers (1963, Special Issue of Plant and Cell Physiology, pp 353-372), the fluorescence changes reflect primarily changes in the redox state of Q(A), the primary quinone electron acceptor of photosystem II (PSII). While it is clearly successful in monitoring the photochemical activity of PSII, a number of important observations cannot be explained within the framework of this simple model. Alternative interpretations have been proposed but were not supported satisfactorily by experimental data. In this review we concentrate on the processes determining the fluorescence rise on a dark-to-light transition and critically analyze the experimental data and the existing models. Recent experiments have provided additional evidence for the involvement of a second process influencing the fluorescence rise once Q(A) is reduced. These observations are best explained by a light-induced conformational change, the focal point of our review. We also want to emphasize that-based on the presently available experimental findings-conclusions on alpha/-centers, PSII connectivity, and the assignment of F-V/F-M to the maximum PSII quantum yield may require critical re-evaluations. At the same time, it has to be emphasized that for a deeper understanding of the underlying physical mechanism(s) systematic studies on light-induced changes in the structure and reaction kinetics of the PSII reaction center are required.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available