Journal
PHOTOSYNTHESIS RESEARCH
Volume 114, Issue 3, Pages 189-206Publisher
SPRINGER
DOI: 10.1007/s11120-012-9788-8
Keywords
C-4 photosynthesis; Light-response curve; PAM fluorescence; Photosynthesis; Quantum yield for photochemistry
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Funding
- Heinz Walz GmbH
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The effect of stepwise increments of red light intensities on pulse-amplitude modulated (PAM) chlorophyll (Chl) fluorescence from leaves of A. thaliana and Z. mays was investigated. Minimum and maximum fluorescence were measured before illumination (F (0) and F (M), respectively) and at the end of each light step ( and , respectively). Calculated values derived from F (0), F (M) and fluorescence according to Oxborough and Baker (1997) were lower than the corresponding measured values. Based on the concept that calculated values are under-estimated because the underlying theory ignores PSI fluorescence, a method was devised to gain relative PSI fluorescence intensities from differences between calculated and measured . This method yields fluorometer-specific PSI data as its input data (F (0), F (M), and ) depend solely on the spectral properties of the fluorometer used. Under the present conditions, the PSI contribution to F (0) fluorescence was 0.24 in A. thaliana and it was independent on the light acclimation status; the corresponding value was 0.50 in Z. mays. Correction for PSI fluorescence affected Z. mays most: the linear relationship between PSI and PSII photochemical yields was clearly shifted toward the one-to-one proportionality line and maximum electron transport was increased by 50 %. Further, correction for PSI fluorescence increased the PSII reaction center-specific parameter, 1/F (0) - 1/F (M), up to 50 % in A. thaliana and up to 400 % in Z. mays.
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