Journal
PHOTOSYNTHESIS RESEARCH
Volume 110, Issue 3, Pages 153-168Publisher
SPRINGER
DOI: 10.1007/s11120-011-9711-8
Keywords
Chloroflexus aurantiacus J-10-fl; Comparative proteomics; Photosynthesis; Liquid chromatography-mass spectrometry
Categories
Funding
- U.S. Department of Energy [DE-AC05-76RL01830]
- Genomic Science Program
- U. S. Department of Energy Office of Biological and Environmental Research (DOE/BER)
- DOE [DE-ACO5-76RLO 1830]
- DOE Office of Basic Energy Sciences [DE-FG02-94ER20137]
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Chloroflexus aurantiacus J-10-fl is a thermophilic green bacterium, a filamentous anoxygenic phototroph, and the model organism of the phylum Chloroflexi. We applied high-throughput, liquid chromatography-mass spectrometry in a global quantitative proteomics investigation of C. aurantiacus cells grown under oxic (chemoorganoheterotrophically) and anoxic (photoorganoheterotrophically) redox states. Our global analysis identified 13,524 high-confidence peptides that matched to 1,286 annotated proteins, 242 of which were either uniquely identified or significantly increased in abundance under photoheterotrophic culture condition. Fifty-four of the 242 proteins are previously characterized photosynthesis-related proteins, including chlorosome proteins, proteins involved in the bacteriochlorophyll biosynthesis, 3-hydroxypropionate (3-OHP) CO2 fixation pathway, and components of electron transport chains. The remaining 188 proteins have not previously been reported. Of these, five proteins were found to be encoded by genes from a novel operon and observed only in photoheterotrophically grown cells. These proteins candidates may prove useful in further deciphering the phototrophic physiology of C. aurantiacus and other filamentous anoxygenic phototrophs.
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