Journal
PHOTOSYNTHESIS RESEARCH
Volume 107, Issue 2, Pages 187-193Publisher
SPRINGER
DOI: 10.1007/s11120-011-9625-5
Keywords
Ultrafiltration; Photosystem-I; Membrane protein; Spinach; Activity
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Funding
- National Natural Science Founds of China [20906104]
- Doctoral Foundation of Shandong Province [BS2009SW029]
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The isolation of photosystem-I (PS-I) from spinach has been conducted using ultrafiltration with 300 kDa molecular weight cut-off polyethersulfone membranes. The effects of ultrafiltration operating conditions on PS-I activity were optimized using parameter scanning ultrafiltration. These conditions included solution pH, ionic strength, stirring speed, and permeate flux. The effects of detergent (Triton X-100 and n-dodecyl-beta-D-maltoside) concentration on time dependent activity of PS-I were also studied using an O-2 electrode. Under optimized conditions, the PS-I purity obtained in the retentate was about 84% and the activity recovery was greater than 94% after ultrafiltration. To our knowledge, this is the first report of the isolation of a membrane protein using ultrafiltration alone.
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