Journal
PHOTOSYNTHESIS RESEARCH
Volume 103, Issue 1, Pages 31-45Publisher
SPRINGER
DOI: 10.1007/s11120-009-9499-y
Keywords
Biotic stress; Chloroplast proteome; Multiprotein families; Nicotiana benthamiana; Pepper mild mottle virus; Two-dimensional gel electrophoresis
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Funding
- Spanish Government [BIO2004-04968-C02-02, BIO2007-67874C02-02, AGL2008-00214]
- FEDER
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We have analyzed the chloroplast proteome of Nicotiana benthamiana using two-dimensional gel electrophoresis and mass spectrometry followed by a database search. In order to improve the resolution of the two-dimensional electrophoresis gels, we have made separate maps for the low and the high pH range. At least 200 spots were detected. We identified 72 polypeptides, some being isoforms of different multiprotein families. In addition, changes in this chloroplast proteome induced by the infection with the Spanish strain of the Pepper mild mottle virus were investigated. Viral infection induced the down-regulation of several chloroplastidic proteins involved in both the photosynthetic electron-transport chain and the Benson-Calvin cycle.
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