Journal
PHOTOSYNTHESIS RESEARCH
Volume 98, Issue 1-3, Pages 277-284Publisher
SPRINGER
DOI: 10.1007/s11120-008-9319-9
Keywords
Manganese complex; Photosystem II; Reconstitution; Water-oxidizing complex; Fluorine-19 NMR
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Funding
- Japan Society for the Promotion of Science (JSPS)
- MEXT, Japan
- JSPS Invitation Fellowship for Research in Japan [S-07106]
- Russian Foundation for Basic Research [08-04-00241, 08-0491300]
- Russian Academy of Sciences
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Reconstitution of Mn-depleted photosystem II (PSII) particles was examined with synthetic trinuclear Mn complexes of newly developed tripod ligands. Rates of the electron transfer and oxygen evolution were up to 74-86 and 52-56% of those measured in native PSII. These values are higher than those for the PSII reconstituted by MnCl(2). The role of the tripod ligands during the reconstitution process was examined by (19)F NMR. Due to the high NMR sensitivity of the (19)F nucleus and the low abundance of fluorine atoms in natural PSII, it was possible to selectively observe the fluorine atoms on the tripod ligand. It was shown that the tripod ligands were released from the Mn complex after the reconstitution. We propose that the primary step in the reconstitution process is the prebinding of the Mn complex to the hydrophobic part of the PSII particle.
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