Journal
PHOTODIAGNOSIS AND PHOTODYNAMIC THERAPY
Volume 10, Issue 3, Pages 320-327Publisher
ELSEVIER
DOI: 10.1016/j.pdpdt.2013.01.007
Keywords
Fullerene; Photosensitizer; Photodynamic inactivation; Candida albicans; Escherichia coli
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Funding
- Consejo Nacional de Investigaciones Cientificas y Tecnicas (CONICET) of Argentina
- SECYT Universidad Nacional de Rio Cuarto
- Agenda Nacional de Promocion Cientifica y Tecnologica (FONCYT)
- CONICET
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The photodynamic mechanism of action induced by N,N-dimethyl-2-(4'-N,N,N-trimethylaminophenyl)fulleropyrrolidinium iodide (DTC602+) was investigated on Candida albicans and Escherichia coli cells. First, photogeneration of superoxide anion radical by DTC602+ in the presence of NADH was detected using nitro blue tetrazolium method in reverse micelles. In C. albicans suspensions, 10 mu M DTC602+ was an effective photosensitizer, producing a similar to 5 log decrease of cell survival when the cultures were irradiated for 30 min with visible light. Also, C. albicans cells growth was not detected in the presence of 10 mu M DTC602+ and irradiation. Photodynamic mechanism investigations were compared in both C. albicans and E. coli cells. Studies under anoxic conditions indicated that oxygen was required for the photodynamic inactivation of these microorganisms. The photocytotoxicity induced by DTC602+ was similar in D2O than in water cell suspensions. Furthermore, photoinactivation of microbial cells was negligible in the presence of azide ion, while the addition of mannitol produced a photoprotective effect on the cellular survival. These results indicate that DTC602+ has potential as agent to the photodynamic inactivation of microbial cells. Also, the photocytotoxicity activity induced by this cationic fullerene derivative can involve the intermediacy of both superoxide anion radical and singlet molecular oxygen. (C) 2013 Elsevier B.V. All rights reserved.
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