4.4 Article

Simultaneous Irradiation with Different Wavelengths of Ultraviolet Light has Synergistic Bactericidal Effect on Vibrio parahaemolyticus

Journal

PHOTOCHEMISTRY AND PHOTOBIOLOGY
Volume 90, Issue 6, Pages 1397-1403

Publisher

WILEY
DOI: 10.1111/php.12309

Keywords

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Funding

  1. Ministry of Education, Culture, Sports, Science and Technology, Japan [26870411, 24500857, 90304047, 09J08670]
  2. Adaptable and Seamless Technology Transfer Program (A-STEP) from the Japan Science and Technology (JST) Agency, Japan
  3. Grants-in-Aid for Scientific Research [26870411, 09J08670, 24500857] Funding Source: KAKEN

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Ultraviolet (UV) irradiation is an increasingly used method of water disinfection. UV rays can be classified by wavelength into UVA (320-400nm), UVB (280-320nm), and UVC (<280nm). We previously developed UVA sterilization equipment with a UVA light-emitting diode (LED). The aim of this study was to establish a new water disinfection procedure using the combined irradiation of the UVA-LED and another UV wavelength. An oxidative DNA product, 8-hydroxy-2'-deoxyguanosine (8-OHdG), increased after irradiation by UVA-LED alone, and the level of cyclobutane pyrimidine dimers (CPDs) was increased by UVC alone in Vibrio parahaemolyticus. Although sequential irradiation of UVA-LED and UVC-induced additional bactericidal effects, simultaneous irradiation with UVA-LED and UVC-induced bactericidal synergistic effects. The 8-OHdG and CPDs production showed no differences between sequential and simultaneous irradiation. Interestingly, the recovery of CPDs was delayed by simultaneous irradiation. The synergistic effect was absent in SOS response-deficient mutants, such as the recA and lexA strains. Because recA- and lexA-mediated SOS responses have crucial roles in a DNA repair pathway, the synergistic bactericidal effect produced by the simultaneous irradiation could depend on the suppression of the CPDs repair. The simultaneous irradiation of UVA-LED and UVC is a candidate new procedure for effective water disinfection.

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