Journal
PHOTOCHEMISTRY AND PHOTOBIOLOGY
Volume 85, Issue 1, Pages 353-357Publisher
WILEY
DOI: 10.1111/j.1751-1097.2008.00447.x
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Funding
- Science and Technology Facilities Council (STFC)
- Rutherford Appleton Laboratory
- University of Salford
- STFC
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Exposure of solutions containing both tryptophan and hydrogen peroxide to a pulsed (similar to 180 fs) laser beam at 750 nm induces luminescence characteristic of 5-hydroxytryptophan. The results indicate that 3-photon excitation of tryptophan results in photoionization within the focal volume of the laser beam. The resulting hydrated electron is scavenged by hydrogen peroxide to produce the hydroxyl radical. The latter subsequently reacts with tryptophan to form 5-hydroxytryptophan. The involvement of hydroxyl radicals is confirmed by the use of ethanol and nitrous oxide as scavengers and their effects on the fluorescence yield in this system. It is postulated that such multiphoton ionization of tryptophanyl residues in cellular proteins may contribute to the photodamage observed during imaging of cells and tissues using multiphoton microscopy.
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