Journal
PHOTOCHEMICAL & PHOTOBIOLOGICAL SCIENCES
Volume 12, Issue 9, Pages 1606-1614Publisher
SPRINGERNATURE
DOI: 10.1039/c3pp50044f
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Funding
- Fundacao para a Ciencia e a Tecnologia (FCT) (SMF) [FCT/SFRH/BPD/34703/2007]
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The fluorescence quenching of protonated beta-carbolines has been investigated in acidic aqueous solutions and in w/o microemulsions using I-, Br-, Cu2+, SCN-, and Pb2+ as quenchers. It was found that fluorescence quenching by these compounds is much more efficient in water than in microemulsions since quenching in microemulsions depends on the simultaneous occupancy of the water droplets by both fluorophore and quencher. Linear Stern-Volmer plots were obtained in all cases, leading to quenching rate constants of ca. 10(8)-10(10) M-1 s(-1) in water and ca. 10(7)-10(8) M-1 s(-1) in microemulsions. In the case of quenching by SCN-, ns flash photolysis studies indicate formation of (SCN)(2)(center dot-) showing that at least part of the quenching process involves an electron transfer mechanism. This indicates that the singlet excited states of the protonated beta-carbolines can act as relatively strong oxidants (E degrees > 1.6 V), capable of oxidizing many species, including the biologically relevant DNA base guanine. The observation of the (SCN)(center dot-) transient in microemulsions demonstrates that it is possible to have the protonated beta-carboline and at least two thiocyanate ions in the same water pool.
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