Journal
PHOTOCHEMICAL & PHOTOBIOLOGICAL SCIENCES
Volume 12, Issue 2, Pages 231-235Publisher
SPRINGERNATURE
DOI: 10.1039/c2pp25254f
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Funding
- Vigoni programme
- University of Parma
- MiUR [PRIN 2008JZ4MLB]
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We introduce a novel fluorescent reporter with potential for super-resolution microscopy, based on the bacterial photoreceptor YtvA. YtvA (from Bacillus subtilis) comprises a photosensitive flavin-based LOV domain, efficiently photo-switchable between fluorescent and non-fluorescent states. We demonstrate Fluorescence PhotoActivation Localization Microscopy (FPALM) studies of live Escherichia coli cells, expressing YtvA molecules.
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