Journal
PHOTOCHEMICAL & PHOTOBIOLOGICAL SCIENCES
Volume 11, Issue 3, Pages 522-532Publisher
SPRINGERNATURE
DOI: 10.1039/c1pp05321c
Keywords
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Funding
- Bundesministerium fur Bildung und Forschung (BMBF) [513, FKZ 13N11066]
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Caged near-IR emitting fluorescent dyes are in high demand in optical microscopy but up to now were unavailable. We discovered that the combination of a carbopyronine dye core and a photosensitive 2-diazo-1-indanone residue leads to masked near-IR emitting fluorescent dyes. Illumination of these caged dyes with either UV or visible light (lambda < 420 nm) efficiently generates fluorescent compounds with absorption and emission at 635 nm and 660 nm, respectively. A high-yielding synthetic route with attractive possibilities for further dye design is described in detail. Good photostability, high contrast, and a large fluorescence quantum yield after uncaging are the most important features of the new compounds for non-invasive imaging in high-resolution optical microscopy. For use in immunolabelling the caged dyes were decorated with a (hydrophilic) linker and an (activated) carboxyl group.
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