Journal
PHOSPHORUS SULFUR AND SILICON AND THE RELATED ELEMENTS
Volume 183, Issue 4, Pages 924-930Publisher
TAYLOR & FRANCIS LTD
DOI: 10.1080/10426500801898366
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- Division Of Chemistry
- Direct For Mathematical & Physical Scien [0744578] Funding Source: National Science Foundation
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The long-sought pathway by which selenocysteyl-tRNA[Ser]Sec is synthesized in eukaryotes has been revealed. Seryl-tRNA[Ser]Sec is O-phosphorylated and SecS, a pyridoxal phosphate-dependent protein, catalyzes the reaction of O-phosphoseryl-tRNA[Ser]Sec with monoselenophosphate to give selenocysteyl-tRNA[Ser]Sec. H-1-{Se-77} HMQC-TOCSY NMR spectroscopy has been developed to detect the selenium-containing amino acids present in selenized yeast after protease XIV digestion. An archived selenized yeast sample is found to contain the novel amino acid S-(methylseleno)cysteine in addition to selenomethionine. Arsenite and selenite react with GSH to form (GS)(2)AsSe-. The structure of this compound has been determined by EXAFS, Se-77 NMR and Raman spectroscopic and chromatographic studies. Its formation under biological conditions has been demonstrated.
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