Journal
PHILOSOPHICAL TRANSACTIONS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES
Volume 369, Issue 1647, Pages -Publisher
ROYAL SOC
DOI: 10.1098/rstb.2013.0337
Keywords
liquid jets; serial femtosecond; crystallography; X-ray free-electron laser
Categories
Funding
- National Science Foundation [MCB 1021557, MCB 1120997]
- BioXFEL Science and Technology Center (NSF) [1231306]
- Direct For Biological Sciences
- Div Of Molecular and Cellular Bioscience [1120997] Funding Source: National Science Foundation
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X-ray free-electron lasers overcome the problem of radiation damage in protein crystallography and allow structure determination from micro-and nanocrystals at room temperature. To ensure that consecutive X-ray pulses do not probe previously exposed crystals, the sample needs to be replaced with the X-ray repetition rate, which ranges from 120 Hz at warm linac-based free-electron lasers to 1 MHz at superconducting linacs. Liquid injectors are therefore an essential part of a serial femtosecond crystallography experiment at an X-ray free-electron laser. Here, we compare different techniques of injecting microcrystals in solution into the pulsed X-ray beam in vacuum. Sample waste due to mismatch of the liquid flow rate to the X-ray repetition rate can be addressed through various techniques.
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