Journal
PHILOSOPHICAL TRANSACTIONS OF THE ROYAL SOCIETY A-MATHEMATICAL PHYSICAL AND ENGINEERING SCIENCES
Volume 371, Issue 1995, Pages -Publisher
ROYAL SOC
DOI: 10.1098/rsta.2012.0525
Keywords
DNA; ruthenium; intercalation
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Funding
- EU LEONARDO programme
- Biotechnology and Biological Sciences Research Council [BB/K019279/1] Funding Source: researchfish
- BBSRC [BB/K019279/1] Funding Source: UKRI
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The crystal structure of the ruthenium DNA 'light-switch' complex Lambda-[Ru(TAP)(2)(11-Cl-dppz)](2+) (TAP= tetraazaphenanthrene, dppz= dipyrido[3, 2-a' : 2',3'-c] phenazine) bound to the oligonucleotide duplex d(TCGGCGCCGA)(2) is reported. The synthesis of the racemic ruthenium complex is described for the first time, and the racemate was used in this study. The crystal structure, at atomic resolution (1.0 angstrom), shows one ligand as a wedge in the minor groove, resulting in the 51 degrees kinking of the double helix, as with the parent Lambda-[Ru(TAP)(2)(dppz)](2+). Each complex binds to one duplex by intercalation of the dppz ligand and also by semi-intercalation of one of the orthogonal TAP ligands into a second symmetrically equivalent duplex. The 11-chloro substituent binds with the major component (66%) oriented with the 11-chloro substituent on the purine side of the terminal step of the duplex.
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