Journal
TUMOR BIOLOGY
Volume 36, Issue 5, Pages 3911-3917Publisher
SPRINGER
DOI: 10.1007/s13277-014-3034-2
Keywords
Under-expression; hFOB1.19; Cellular apoptosis; Cellular invasion; Luciferase reporter assay
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Funding
- Fundamental Research Funds for the Central Universities [21614308]
- Medical Science Research Foundation of Guangdong Province [B2012195]
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MicroRNAs (miRNAs) are essential to the progression of osteosarcoma. Previous research using osteosarcoma samples confirmed that miR-377 expression is less than that observed in normal human osteoblast expression. These data suggest a role for miR-377 in osteosarcoma that warrants investigation. To address this concept, we measured miR-377 expression in two cell models, and we also observed that miR-377 was less expressed in osteosarcoma MG-63 cells compared to the hFOB1.19 human fetal osteoblastic cell line. Moreover, miR-377 overexpression reduced cell proliferation and suppressed invasion of MG-63 cells but had no effect on MG-63 cell apoptosis. Because cyclin-dependent kinase 6 (CDK6) may be a potential target of miR-377 in osteosarcoma cells, we overexpressed CDK6 and observed that overexpression attenuated tumor suppressive effects of miR-377 on cell proliferation. Our data suggest that miR-377 can suppress proliferation in MG-63 cells in part by targeting CDK6.
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